The Human IL-7 (Interleukin-7) CLIA Kit is a top-of-the-line assay kit designed for the accurate measurement of IL-7 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, allowing for precise and dependable results that are perfect for a wide variety of research applications.IL-7 is a critical cytokine involved in immune system regulation and cell development, making it a key player in various diseases such as cancer, autoimmune disorders, and infectious diseases.
By accurately measuring IL-7 levels, researchers can gain valuable insights into disease progression and potential treatment options.Overall, the Human IL-7 CLIA Kit is a reliable tool for researchers looking to study the role of IL-7 in various physiological and pathological processes, making it an indispensable asset in the field of biomedical research.
Product Name:
Human IL-7 CLIA Kit
SKU:
HUES00428
Target:
Human IL-7
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-7. You can calculate the concentration of Human IL-7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
89-104
102-116
Average (%)
92
96
110
1:4
Range (%)
93-108
91-105
89-104
Average (%)
100
98
96
1:8
Range (%)
97-108
99-112
86-98
Average (%)
102
105
91
1:16
Range (%)
101-112
88-104
92-107
Average (%)
106
95
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
89-103
95
Cell culture media (n=5)
90-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
26.91
48.51
179.67
28.32
50.41
182.64
Standard deviation
3.09
5.72
10.96
3.45
6.0
15.93
C V (%)
11.48
11.79
6.1
12.18
11.9
8.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-7 in samples. No significant cross-reactivity or interference between Human IL-7 and analogues was observed.
