The Human IL-8 Colorimetric CLIA Kit is specifically designed for the quantitative measurement of Interleukin 8 (IL-8) levels in human biological samples such as serum, plasma, and cell culture supernatants. IL-8 is a pro-inflammatory cytokine that plays a critical role in the immune response and is involved in various physiological and pathological processes.This CLIA kit offers high sensitivity and specificity, ensuring accurate and reliable results for research purposes.
IL-8 is known to be a key mediator in inflammatory diseases, cancer, and immune responses, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human IL-8 Colorimetric CLIA Kit is a valuable tool for researchers looking to study the role of IL-8 in various disease states and develop new treatment strategies.
Product Name:
Human IL-8 CLIA Kit
SKU:
HUES00047
Target:
Human IL-8
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-8. You can calculate the concentration of Human IL-8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
87-101
91-105
Average (%)
95
92
96
1:4
Range (%)
100-116
101-116
88-99
Average (%)
108
107
93
1:8
Range (%)
91-105
94-109
101-116
Average (%)
97
100
106
1:16
Range (%)
97-112
94-106
94-107
Average (%)
104
100
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
100
EDTA plasma (n=5)
100-115
107
Cell culture media (n=5)
99-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.63
106.84
411.53
48.85
107.69
421.0
Standard deviation
4.97
11.51
28.64
5.72
8.63
28.5
C V (%)
9.82
10.77
6.96
11.71
8.01
6.77
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-8 in samples. No significant cross-reactivity or interference between Human IL-8 and analogues was observed.
