The Human IL-9 CLIA Kit is a powerful tool for measuring IL-9 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it an indispensable resource for research in a variety of fields.IL-9 is a key regulator of immune responses, playing a critical role in the development and function of various immune cells. Dysregulation of IL-9 has been associated with inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
Whether you're exploring the role of IL-9 in allergic responses, autoimmune disorders, or other immunological processes, the Human IL-9 CLIA Kit from Assaygenie provides the precision and reliability you need for your research. Order yours today and unlock new insights into the complex world of immune regulation.
Product Name:
Human IL-9 CLIA Kit
SKU:
HUES00154
Target:
Human IL-9
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IL-9. You can calculate the concentration of Human IL-9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
100-112
98-115
Average (%)
106
106
106
1:4
Range (%)
93-106
87-103
102-117
Average (%)
98
94
108
1:8
Range (%)
85-99
99-113
97-113
Average (%)
92
106
105
1:16
Range (%)
103-118
99-116
87-99
Average (%)
110
106
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
91-105
98
Cell culture media (n=5)
93-103
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.43
126.02
289.97
34.36
127.42
318.64
Standard deviation
4.35
9.7
24.47
3.05
12.59
31.19
C V (%)
11.94
7.7
8.44
8.88
9.88
9.79
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human IL-9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-9 in samples. No significant cross-reactivity or interference between Human IL-9 and analogues was observed.
