The Human IL-9 ELISA Kit is a powerful tool for measuring IL-9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is designed for high sensitivity and specificity, providing researchers with accurate and reliable results for a variety of applications.IL-9 is a cytokine that plays a key role in immune responses, particularly in allergic reactions, asthma, and autoimmune diseases.
By accurately measuring IL-9 levels, researchers can gain a better understanding of the immune system and develop targeted treatments for these conditions.Whether studying the role of IL-9 in disease pathology or evaluating the effectiveness of potential therapies, the Human IL-9 ELISA Kit is a valuable tool for researchers looking to advance their knowledge in immunology and inflammation.
Product Name:
Human IL-9 ELISA Kit
SKU:
HUES01447
Target:
Human IL-9
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-9. You can calculate the concentration of Human IL-9 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
92-104
91-104
Average (%)
90
98
98
1:4
Range (%)
96-114
84-95
93-106
Average (%)
104
89
99
1:8
Range (%)
96-110
81-93
99-115
Average (%)
101
87
105
1:16
Range (%)
95-110
88-102
96-109
Average (%)
101
93
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
97
EDTA plasma (n=5)
93-105
100
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.1
135.7
409.2
46.4
133.9
412.5
Standard deviation
3
6.9
18.4
3.0
6.2
13.6
C V (%)
5.87
5.08
4.5
6.47
4.63
3.3
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IL-9 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IL-9 in samples. No significant cross-reactivity or interference between Human IL-9 and analogues was observed.
