The Human INHA (Inhibin A) CLIA Kit is a powerful tool for measuring levels of Inhibin A in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reliable results for various research applications.Inhibin A is a key protein involved in inhibiting follicle-stimulating hormone (FSH) production, playing a critical role in the regulation of reproductive processes. Abnormal levels of Inhibin A have been linked to conditions such as infertility, ovarian cancer, and polycystic ovary syndrome, making it an important biomarker for studying these diseases and developing potential treatments.
Overall, the Human INHA CLIA Kit offers researchers a valuable tool for investigating the role of Inhibin A in various physiological and pathological processes, paving the way for new discoveries and advancements in reproductive health and disease research.
Product Name:
Human INHA (Inhibin A) CLIA Kit
SKU:
HUES00184
Target:
Human INHA (Inhibin A)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INHA. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INHA, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human INHA. You can calculate the concentration of Human INHA in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-111
89-105
98-109
Average (%)
101
96
104
1:4
Range (%)
101-116
98-115
100-118
Average (%)
108
106
109
1:8
Range (%)
86-98
103-118
89-105
Average (%)
91
110
96
1:16
Range (%)
92-108
83-97
100-117
Average (%)
100
90
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-97
90
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
98-113
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
25.48
78.58
201.67
27.26
85.32
219.57
Standard deviation
3.2
7.61
14.26
2.9
6.79
17.13
C V (%)
12.56
9.68
7.07
10.64
7.96
7.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human INHA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INHA in samples. No significant cross-reactivity or interference between Human INHA and analogues was observed.
