The Human INSL3 (Insulin-like Protein 3) CLIA Kit is specifically designed for the precise measurement of INSL3 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it a valuable tool for various research applications.INSL3 is a key hormone involved in reproductive health, playing a crucial role in testicular descent and development.
Dysregulation of INSL3 has been linked to conditions such as cryptorchidism and male infertility, making it an important biomarker for studying these disorders and potentially developing targeted treatments.Overall, the Human INSL3 CLIA Kit offers researchers a reliable and efficient method for quantifying INSL3 levels, allowing for a better understanding of its biological functions and potential implications in various health conditions.
Product Name:
Human INSL3 (Insulin Like Protein 3) CLIA Kit
SKU:
HUES00290
Target:
Human INSL3 (Insulin Like Protein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INSL3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INSL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSL3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human INSL3. You can calculate the concentration of Human INSL3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-119
90-102
91-106
Average (%)
110
96
98
1:4
Range (%)
98-117
101-116
97-113
Average (%)
107
109
105
1:8
Range (%)
88-98
94-106
99-116
Average (%)
93
100
106
1:16
Range (%)
87-102
101-114
86-99
Average (%)
94
106
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-114
104
EDTA plasma (n=5)
92-107
100
Cell culture media (n=5)
93-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.88
281.99
851.44
111.83
295.88
861.62
Standard deviation
13.02
22.73
65.48
12.2
25.8
96.16
C V (%)
12.3
8.06
7.69
10.91
8.72
11.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human INSL3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INSL3 in samples. No significant cross-reactivity or interference between Human INSL3 and analogues was observed.
