The Human INSL5 (Insulin-Like Protein 5) CLIA Kit is a powerful tool for the accurate measurement of INSL5 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.INSL5 is a unique insulin-like protein that plays a critical role in metabolic regulation and has been implicated in various diseases such as obesity and inflammatory disorders.
By accurately quantifying INSL5 levels, researchers can gain valuable insights into the physiological function of this protein and its potential role in disease pathogenesis.With its user-friendly protocol and consistent performance, the Human INSL5 CLIA Kit is an essential tool for researchers studying the role of INSL5 in health and disease. Stay ahead in your research with this cutting-edge kit from AssayGenie.
Product Name:
Human INSL5 (Insulin Like Protein 5) CLIA Kit
SKU:
HUES00291
Target:
Human INSL5 (Insulin Like Protein 5)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INSL5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INSL5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSL5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human INSL5. You can calculate the concentration of Human INSL5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
100-118
100-113
Average (%)
106
107
107
1:4
Range (%)
92-110
91-102
95-108
Average (%)
100
97
100
1:8
Range (%)
101-115
86-98
91-103
Average (%)
108
91
96
1:16
Range (%)
89-100
86-97
99-112
Average (%)
95
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
108
EDTA plasma (n=5)
87-102
93
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
102.94
303.87
796.74
109.2
327.9
718.54
Standard deviation
9.66
34.58
78.48
13.87
23.87
59.35
C V (%)
9.38
11.38
9.85
12.7
7.28
8.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human INSL5 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INSL5 in samples. No significant cross-reactivity or interference between Human INSL5 and analogues was observed.
