The Human INSR (Insulin Receptor) CLIA Kit is a highly reliable and sensitive assay designed for the precise detection of insulin receptor levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional specificity and accuracy, ensuring consistent and reproducible results that are vital for various research applications.The insulin receptor is a key protein involved in regulating glucose metabolism and cell growth, making it a crucial target for understanding metabolic disorders like diabetes and obesity.
By measuring insulin receptor levels, researchers can gain valuable insights into insulin signaling pathways and potential therapeutic interventions for related diseases.With its superior performance and ease of use, the Human INSR (Insulin Receptor) CLIA Kit is an essential tool for investigating insulin receptor function and its implications in health and disease. Trust in this kit for reliable data and impactful discoveries in the field of metabolic research.
Product Name:
Human INSR (Insulin Receptor) CLIA Kit
SKU:
HUES00326
Target:
Human INSR (Insulin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INSR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INSR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human INSR. You can calculate the concentration of Human INSR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
102-113
87-102
Average (%)
105
108
94
1:4
Range (%)
93-105
96-110
100-113
Average (%)
98
104
106
1:8
Range (%)
88-102
103-120
85-97
Average (%)
94
109
90
1:16
Range (%)
91-108
88-102
100-118
Average (%)
99
95
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-117
107
EDTA plasma (n=5)
96-113
103
Cell culture media (n=5)
98-116
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.2
8.26
1.05
2.25
7.93
Standard deviation
0.12
0.2
0.81
0.11
0.2
0.64
C V (%)
12.37
9.09
9.81
10.48
8.89
8.07
Sample type &Sample volume:
Tissue homogenates,cell lysates and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human INSR concentrations in Tissue homogenates,cell lysates and other biological fluids.
Specificity:
This kit recognizes Human INSR in samples. No significant cross-reactivity or interference between Human INSR and analogues was observed.
