The Human INSR (Insulin Receptor) ELISA Kit is specifically designed for the precise measurement of insulin receptor levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research purposes.The insulin receptor is a key protein involved in regulating glucose uptake and metabolism in the body. Dysfunction of the insulin receptor is associated with conditions such as diabetes, metabolic syndrome, and obesity.
Therefore, precise measurement of insulin receptor levels is essential for understanding the mechanisms underlying these conditions and developing targeted therapies.With its high-quality components and user-friendly design, the Human INSR (Insulin Receptor) ELISA Kit is a valuable tool for researchers studying insulin signaling pathways and metabolic disorders. Get reliable and reproducible data for your studies with this advanced ELISA kit from Assay Genie.
Product Name:
Human INSR (Insulin Receptor) ELISA Kit
SKU:
HUES01661
Target:
Human INSR (Insulin Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INSR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INSR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INSR. You can calculate the concentration of Human INSR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
86-98
96-112
Average (%)
95
93
102
1:4
Range (%)
92-108
85-98
90-101
Average (%)
98
92
95
1:8
Range (%)
90-104
86-97
84-96
Average (%)
98
91
89
1:16
Range (%)
87-101
82-93
86-98
Average (%)
93
88
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
93-105
98
Cell culture media (n=5)
86-98
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.64
11.66
49.95
4.62
11.01
48.27
Standard deviation
0.26
0.47
2.08
0.31
0.62
1.47
C V (%)
5.6
4.03
4.16
6.71
5.63
3.05
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human INSR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human INSR in samples. No significant cross-reactivity or interference between Human INSR and analogues was observed.
