Description
| Product Name: | Human Insulin ELISA Kit |
| SKU: | HDES0114 |
| Size: | 96T |
| Target: | INS |
| Detection Method: | Sandwich ELISA |
| Result Type: | Quantitative: Sensitivity 99.3%, Specificity 99.8% |
| Sample Type: | Serum |
| Assay Time: | 1 h 15 min |
| Detection Wavelength: | 450/630nm |
| Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the principle to detect the Tuberculosis (TB) IgG in human serum, www.vetassay-elab.com 2 Enzyme linked immunosorbent assay (ELISA) sandwich method was used to detect the content of human insulin in serum. Firstly, a monoclonal antibody is coated onto a microplate to prepare a solid-phase antibody. Then, the serum to be tested and another monoclonal antibody labeled with horseradish peroxidase are added to form a complex of coated antibody insulin labeled antibody. After color development, the absorbance value (OD value) is measured using an enzyme-linked immunosorbent assay (ELISA) reader. The concentration absorbance curve is fitted using a computer or graph to calculate the human insulin content in the serum to be tested.
