Human Insulin Like Growth Factor 2 (IGF2) ELISA Kit
The Human Insulin-like Growth Factor 2 (IGF2) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of IGF2 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an essential tool for a wide range of research applications.IGF2 is a crucial growth factor that plays a significant role in cell growth, proliferation, and survival. Dysregulation of IGF2 has been associated with various diseases, including cancer, metabolic disorders, and developmental anomalies.
By accurately measuring IGF2 levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.The Human IGF2 ELISA Kit offers a user-friendly protocol, a wide dynamic range, and high sensitivity, making it a valuable tool for studying the role of IGF2 in health and disease. Whether investigating basic biological mechanisms or developing novel therapies, this kit provides the precision and accuracy needed for impactful research outcomes.
Product Name:
Human Insulin Like Growth Factor 2 (IGF2) ELISA Kit
Product Code:
HUDL01445
Size:
96 Assays
Target:
IGF2
Synonyms:
INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
