Human Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) ELISA Kit
The Human Insulin-like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) ELISA Kit is a valuable tool for the precise measurement of IGF2BP3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.IGF2BP3, also known as IMP3, is a key protein involved in the regulation of mRNA binding and stability, playing a crucial role in various cellular processes including tumorigenesis and metastasis.
Aberrant expression of IGF2BP3 has been linked to several cancers, making it a promising biomarker for cancer research and potential therapeutic targets.With its high-performance capabilities, the Human IGF2BP3 ELISA Kit is a valuable tool for researchers studying the role of IGF2BP3 in cancer biology and other diseases. Its reliability and precision make it an essential component of any laboratory seeking to investigate the functions and implications of IGF2BP3 in human health and disease.
Product Name:
Human Insulin Like Growth Factor 2 mRNA Binding Protein 3 (IGF2BP3) ELISA Kit
Product Code:
HUDL01444
Size:
96 Assays
Target:
IGF2BP3
Synonyms:
MP-3, IMP3, KOC1, VICKZ3, CT98, IGF II mRNA Binding Protein 3, Cancer/Testis Antigen 98, KH domain-containing protein overexpressed in cancer
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
