Human Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit
The Human Insulin-Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit. This kit is specially designed for the precise detection of IGFBP1 levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reliable results, making it a valuable tool for various research applications.IGFBP1 is an important protein that plays a key role in regulating insulin-like growth factor signaling and is implicated in various physiological processes, including cell growth and proliferation.
Dysregulation of IGFBP1 has been associated with several health conditions, making it a promising biomarker for studying diseases such as diabetes, cancer, and growth disorders.With its innovative design and advanced technology, the Human Insulin-Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit provides researchers with a powerful tool for investigating the role of IGFBP1 in physiological and pathological processes, ultimately contributing to the development of potential diagnostic and therapeutic strategies.
Product Name:
Human Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit
Product Code:
HUDL01448
Size:
96 Assays
Target:
IGFBP1
Synonyms:
IBP1 AFBP IGF-BP25 PP12 Placental Protein 12 Amniotic Fluid Binding Protein Alpha-Pregnancy-Associated Endometrial Globulin Growth Hormone Independent-Bind
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
