Human Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
The Human Insulin-like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit is specially designed for the precise measurement of IGFBP3 levels in human serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.IGFBP3 is a key protein that binds to insulin-like growth factors, regulating their activity and promoting cell growth and survival. It plays a crucial role in various biological processes, including cell proliferation, differentiation, and apoptosis.
Dysregulation of IGFBP3 has been linked to various diseases, such as cancer, diabetes, and growth disorders, making it a valuable biomarker for disease diagnosis and monitoring.With the Human Insulin-like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit, researchers can confidently analyze IGFBP3 levels in samples and gain valuable insights into its role in health and disease. This kit is a valuable tool for investigating IGFBP3 biology and developing potential therapeutic strategies for related conditions.
Product Name:
Human Insulin Like Growth Factor Binding Protein 3 (IGFBP3) ELISA Kit
Product Code:
HUDL01450
Size:
96 Assays
Target:
IGFBP3
Synonyms:
BP53, IBP3, Growth Hormone-Dependent Binding Protein, Acid Stable Subunit Of The 140 K IGF Complex
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
