The Human Interferon Alpha 2 (IFNA2) ELISA Kit is specifically designed for the accurate quantification of IFNA2 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.Interferon Alpha 2 is a vital cytokine involved in the immune response, playing a crucial role in antiviral defense and immune regulation. Aberrant IFNA2 levels have been linked to various diseases, including viral infections, autoimmune disorders, and certain types of cancer.
As such, this ELISA kit serves as a valuable tool for researchers looking to investigate the role of IFNA2 in disease pathogenesis and potential therapeutic interventions.Overall, the Human Interferon Alpha 2 (IFNA2) ELISA Kit offers reliable and accurate measurement of IFNA2 levels, making it an indispensable resource for those studying the complex interplay between the immune system and disease processes.
Product Name:
Human Interferon Alpha 2 (IFNa2) ELISA Kit
Product Code:
HUDL01436
Size:
96 Assays
Target:
IFNa2
Synonyms:
IFNA2A, IFNA2B, IFNA2C, LeIF A, Alpha-2a Interferon, Interferon Alpha 2b, Interferon Alpha A
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
