Human Interferon Alpha/Beta Receptor 2 (IFNa/bR2) ELISA Kit
The Human Interferon Alpha/Beta Receptor 2 (IFNAR2) ELISA Kit is specifically designed for the precise measurement of IFNAR2 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an excellent tool for a wide range of research applications.IFNAR2 is a critical receptor involved in the immune response to viral infections, playing a crucial role in signaling pathways that are activated by interferons. By accurately measuring IFNAR2 levels, researchers can gain valuable insights into the immune system's response to viral threats, leading to a better understanding of diseases such as viral infections and autoimmune disorders.
Additionally, the IFNAR2 ELISA Kit can also be used to monitor the effectiveness of potential therapeutics targeting the interferon signaling pathway.Overall, the Human Interferon Alpha/Beta Receptor 2 (IFNAR2) ELISA Kit is an essential tool for researchers studying immunology, virology, and autoimmune diseases, providing accurate and reliable data for their research projects.
Product Name:
Human Interferon Alpha/Beta Receptor 2 (IFNa/bR2) ELISA Kit
Product Code:
HUDL01439
Size:
96 Assays
Target:
IFNa/bR2
Synonyms:
IFNAR2, IFN-alpha-REC, IFN-R, IFNABR, IFNARB, Interferon(Alpha,Beta And Omega)Receptor Beta Chain, Interferon alpha binding protein, Type I interferon receptor 2
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
