Human Interferon Gamma Inducible Protein 16 (IFI16) ELISA Kit
The Human Interferon Gamma Inducible Protein 16 (IFI16) ELISA Kit is a versatile and reliable tool for the quantitative detection of IFI16 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, offering accurate and consistent results for various research applications.IFI16 is a key protein involved in the regulation of immune responses and inflammation. It plays a crucial role in the innate immune system, detecting intracellular DNA and activating pathways that help in the defense against pathogens. Dysregulation of IFI16 has been linked to various autoimmune diseases, viral infections, and inflammatory conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With this ELISA kit, researchers can easily analyze IFI16 levels in human samples, gaining valuable insights into immune responses, inflammatory processes, and disease mechanisms. Its reliable performance and user-friendly design make it an essential tool for laboratories conducting research in immunology, infectious diseases, and inflammatory disorders.
Product Name:
Human Interferon Gamma Inducible Protein 16 (IFI16) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
