Description
Human Interferon regulatory factor 8 (IRF8) ELISA Kit
The Human Interferon Regulatory Factor 8 (IRF8) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of IRF8 levels in human serum, plasma, and cell culture supernatants. This kit provides accurate and reliable results, making it suitable for a wide range of research applications.IRF8 is a key transcription factor involved in immune response regulation, particularly in the differentiation and function of immune cells such as macrophages and dendritic cells.
Dysregulation of IRF8 has been implicated in various autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a potential therapeutic target and biomarker for disease diagnosis and prognosis.With its high performance characteristics and easy-to-use protocol, the Human IRF8 ELISA Kit is an essential tool for researchers studying the role of IRF8 in immune regulation and its implications in disease pathogenesis.
| Product Name: | Human Interferon regulatory factor 8 (IRF8) ELISA Kit |
| SKU: | HUEB1941 |
| Size: | 96T |
| Target: | Human Interferon regulatory factor 8 (IRF8) |
| Synonyms: | Interferon consensus sequence-binding protein, H-ICSBP, IRF-8, ICSBP1 |
| Assay Type: | Sandwich |
| Detection Method: | ELISA |
| Reactivity: | Human |
| Detection Range: | 31.2-2000pg/mL |
| Sensitivity: | 15.61pg/mL |
| Intra CV: | 6.8% | ||||||||||||||||||||
| Inter CV: | 8.4% | ||||||||||||||||||||
| Linearity: |
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| Recovery: |
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| Function: | Plays a role as a transcriptional activator or repressor (PubMed:25122610). Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes. |
| Uniprot: | Q02556 |
| Sample Type: | Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids |
| Specificity: | Natural and recombinant human Interferon regulatory factor 8 |
| Sub Unit: | Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with COPS2. |
| Research Area: | Immunology |
| Subcellular Location: | Nucleus Cytoplasm In resting macrophages, localizes in the cytoplasm. Translocated in the nucleus upon IFN-gamma induction. |
| Storage: | Please see kit components below for exact storage details |
| Note: | For research use only |
| UniProt Protein Function: | IRF8: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with COPS2. By IFNG/IFN-gamma. Predominantly expressed in lymphoid tissues. Belongs to the IRF family. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 16q24.1 Cellular Component: nucleoplasm; cytosol Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding Biological Process: transcription from RNA polymerase II promoter; positive regulation of interferon-gamma production; positive regulation of interleukin-12 production; cytokine and chemokine mediated signaling pathway; defense response to bacterium; myeloid cell differentiation; immune response; negative regulation of transcription from RNA polymerase II promoter; defense response to protozoan; phagocytosis Disease: Monocyte And Dendritic Cell Deficiency, Autosomal Recessive; Immunodeficiency 32a |
| NCBI Summary: | Interferon consensus sequence-binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA-binding domain in the N-terminal region and a divergent C-terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN-stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN-alpha and IFN-beta. IRF family proteins also control expression of IFN-alpha and IFN-beta-regulated genes that are induced by viral infection. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q02556 |
| NCBI GenInfo Identifier: | 6016308 |
| NCBI Gene ID: | 3394 |
| NCBI Accession: | Q02556.2 |
| UniProt Secondary Accession: | Q02556,A0AV82, |
| UniProt Related Accession: | Q02556 |
| Molecular Weight: | Calculated MW: 48kDaObserved MW: 53kDa |
| NCBI Full Name: | Interferon regulatory factor 8 |
| NCBI Synonym Full Names: | interferon regulatory factor 8 |
| NCBI Official Symbol: | IRF8 |
| NCBI Official Synonym Symbols: | ICSBP; IRF-8; ICSBP1; IMD32A; IMD32B; H-ICSBP |
| NCBI Protein Information: | interferon regulatory factor 8; interferon consensus sequence binding protein 1 |
| UniProt Protein Name: | Interferon regulatory factor 8 |
| UniProt Synonym Protein Names: | Interferon consensus sequence-binding protein; H-ICSBP; ICSBP |
| Protein Family: | Interferon regulatory factor |
| UniProt Gene Name: | IRF8 |
| UniProt Entry Name: | IRF8_HUMAN |
| Component | Quantity (96 Assays) | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | -20°C |
| Lyophilized Standard | 2 | -20°C |
| Sample Diluent | 20ml | -20°C |
| Assay Diluent A | 10mL | -20°C |
| Assay Diluent B | 10mL | -20°C |
| Detection Reagent A | 120µL | -20°C |
| Detection Reagent B | 120µL | -20°C |
| Wash Buffer | 30mL | 4°C |
| Substrate | 10mL | 4°C |
| Stop Solution | 10mL | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
*Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37°C directly). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at -20°C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their experiments. We recommend running all samples in duplicate.
| Step | |
| 1. | Add Sample: Add 100µL of Standard, Blank, or Sample per well. The blank well is added with Sample diluent. Solutions are added to the bottom of micro ELISA plate well, avoid inside wall touching and foaming as possible. Mix it gently. Cover the plate with sealer we provided. Incubate for 120 minutes at 37°C. |
| 2. | Remove the liquid from each well, don't wash. Add 100µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Gently tap the plate to ensure thorough mixing. Incubate for 1 hour at 37°C. Note: if Detection Reagent A appears cloudy warm to room temperature until solution is uniform. |
| 3. | Aspirate each well and wash, repeating the process three times. Wash by filling each well with Wash Buffer (approximately 400µL) (a squirt bottle, multi-channel pipette,manifold dispenser or automated washer are needed). Complete removal of liquid at each step is essential. After the last wash, completely remove remaining Wash Buffer by aspirating or decanting. Invert the plate and pat it against thick clean absorbent paper. |
| 4. | Add 100µL of Detection Reagent B working solution to each well. Cover with the Plate sealer. Incubate for 60 minutes at 37°C. |
| 5. | Repeat the wash process for five times as conducted in step 3. |
| 6. | Add 90µL of Substrate Solution to each well. Cover with a new Plate sealer and incubate for 10-20 minutes at 37°C. Protect the plate from light. The reaction time can be shortened or extended according to the actual color change, but this should not exceed more than 30 minutes. When apparent gradient appears in standard wells, user should terminatethe reaction. |
| 7. | Add 50µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing. |
| 8. | Determine the optical density (OD value) of each well at once, using a micro-plate reader set to 450 nm. User should open the micro-plate reader in advance, preheat the instrument, and set the testing parameters. |
| 9. | After experiment, store all reagents according to the specified storage temperature respectively until their expiry. |
When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
| Sample Type | Protocol |
| Serum | If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. |
| Plasma | Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit. |
| Urine & Cerebrospinal Fluid | Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid. |
| Cell culture supernatant | Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately. |
| Cell lysates | Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Tissue homogenates | The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C. |
| Tissue lysates | Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C. |
| Breast Milk | Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles. |
