The Human Interleukin-1 Eta (IL1H) ELISA Kit is a reliable and accurate assay designed for the detection of IL1H levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for your research needs.Interleukin-1 Eta (IL1H) is a cytokine involved in various inflammatory responses and immune system regulation. Its dysregulation has been linked to inflammatory diseases and autoimmune disorders, making it a valuable biomarker for studying these conditions and evaluating potential therapeutic interventions.
By using the Human Interleukin-1 Eta (IL1H) ELISA Kit, researchers can effectively measure IL1H levels in human samples, providing valuable insights into the role of this cytokine in health and disease. Whether studying inflammation, immune responses, or therapeutic targets, this ELISA kit is an essential tool for accurate and reliable quantification of IL1H levels in various biological samples.
Product Name:
Human Interleukin 1 Eta (IL-1h) ELISA Kit
Product Code:
HUDL01486
Size:
96 Assays
Target:
IL1h
Synonyms:
IL1F8, IL36b, FIL1, FIL1-(ETA), FIL1H, IL-1F8, IL-1H2, IL1-ETA, IL1H2, Interleukin 36, Beta, Interleukin-1 Family Member 8, Interleukin-1 homolog 2
Detection Method:
Sandwich
Range:
15.625-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
