Human Interleukin 1 Receptor Accessory Protein (IL-1RAP) ELISA Kit
The Human Interleukin-1 Receptor Accessory Protein (IL1RAP) ELISA Kit is a reliable and accurate tool for detecting IL1RAP levels in human biological samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reproducible results, making it suitable for a wide range of research applications.IL1RAP is a critical receptor accessory protein involved in the interleukin-1 signaling pathway, playing a key role in inflammation and immune response regulation. Dysregulation of IL1RAP has been linked to various inflammatory conditions, autoimmune diseases, and cancer, making it a valuable biomarker for studying these diseases and developing therapeutic strategies.
By using the Human IL1RAP ELISA Kit, researchers can accurately measure IL1RAP levels in human samples, facilitating a better understanding of its role in disease pathogenesis and progression. This kit provides a convenient and effective means of studying IL1RAP function and its potential as a target for novel therapeutic interventions.
Product Name:
Human Interleukin 1 Receptor Accessory Protein (IL-1RAP) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
