Human Interleukin 12 Receptor Beta 2 (IL-12Rb2) ELISA Kit
The Human Interleukin-12 Receptor Beta 2 (IL12RB2) ELISA Kit is specially designed for the accurate measurement of IL12RB2 levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers precise and reproducible results, making it an invaluable tool for various research applications.IL12RB2 is a crucial receptor protein involved in the immune response, particularly in regulating T cell function and cytokine signaling. Dysregulation of IL12RB2 has been linked to various autoimmune diseases, inflammatory disorders, and infectious diseases, highlighting its importance as a potential therapeutic target and diagnostic marker.
By providing accurate quantification of IL12RB2 levels, the Human IL12RB2 ELISA Kit empowers researchers to gain deeper insights into immune system modulation, disease pathogenesis, and potential treatment strategies. Trust in this kit for reliable and meaningful results in your studies on immune responses and inflammatory conditions.
Product Name:
Human Interleukin 12 Receptor Beta 2 (IL-12Rb2) ELISA Kit
Product Code:
HUDL01466
Size:
96 Assays
Target:
IL12Rb2
Synonyms:
IL12R-B2, IL12-RB2, RP11-102M16.1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-99
91
EDTA plasma (n=5)
80-100
90
Heparin plasma (n=5)
82-99
90
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
