The Human Interleukin-25 (IL-25) ELISA Kit is specifically designed for the precise measurement of interleukin-25 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit allows for accurate and reproducible results, making it an excellent choice for a variety of research applications.Interleukin-25, also known as IL-17E, is a cytokine that plays a crucial role in regulating inflammatory responses and immune system function. It has been implicated in conditions such as asthma, inflammatory bowel disease, and allergic responses, making it a valuable biomarker for studying these diseases and potential therapeutic targets.
By utilizing the Human Interleukin-25 (IL-25) ELISA Kit, researchers can accurately quantify IL-25 levels in biological samples, allowing for a better understanding of its role in various disease states and potential therapeutic interventions. This kit is a valuable tool for researchers looking to further explore the functions and implications of interleukin-25 in human health and disease.
Product Name:
Human Interleukin 25 (IL-25) ELISA Kit
Product Code:
HUDL01501
Size:
96 Assays
Target:
IL25
Synonyms:
IL17E, IL17-E, Interleukin-17E
Detection Method:
Sandwich
Range:
15.62-1000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
