The Human Irisin ELISA Kit is meticulously crafted for the precise and quantitative detection of Irisin levels in various biological samples of human origin. Irisin, a myokine released during physical activity, plays a crucial role in energy metabolism, thermogenesis, and potential regulation of adipose tissue browning, providing valuable insights into exercise physiology and metabolic health. Our ELISA kit offers exceptional sensitivity and specificity to ensure accurate and reproducible results in your research endeavors.
Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for investigating the role of Irisin in various physiological and metabolic processes. Trust Assay Genie's ELISA kit for dependable quantification and comprehensive analysis of Irisin biomarkers in your studies.
Product Name:
Human Irisin ELISA Kit
SKU:
AEES00150
Target:
Human Irisin
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Irisin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Irisin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Irisin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Irisin. You can calculate the concentration of Human Irisin in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-106
99-110
95-102
Average (%)
103
102
98
1:4
Range (%)
89-96
96-107
92-104
Average (%)
93
99
96
1:8
Range (%)
89-98
96-107
89-103
Average (%)
95
103
96
1:16
Range (%)
97-106
97-106
89-98
Average (%)
100
102
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-101
93
EDTA plasma (n=5)
94-109
102
Cell culture media (n=5)
93-102
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.28
104.39
474.47
42.15
100.83
403.9
Standard deviation
2.15
5.18
23.82
2.15
4.59
18.42
C V (%)
5.34
4.96
5.02
5.1
4.55
4.56
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human Irisin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human Irisin in samples. No significant cross-reactivity or interference between Human Irisin and analogues was observed.
