Human IRS1 (Insulin Receptor Substrate 1) ELISA Kit
The Human IRS1 (Insulin Receptor Substrate 1) ELISA Kit is specifically designed for the quantitative measurement of IRS1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, providing accurate and consistent results for various research applications.IRS1 is a key protein in the insulin signaling pathway, playing a crucial role in regulating glucose metabolism and cell growth.
Dysregulation of IRS1 has been linked to insulin resistance, diabetes, and other metabolic disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.With its reliable performance and ease of use, the Human IRS1 ELISA Kit is an essential tool for researchers studying the intricate mechanisms of insulin signaling and its implications in various diseases.
Product Name:
Human IRS1 (Insulin Receptor Substrate 1) ELISA Kit
SKU:
HUES03530
Target:
Human IRS1 (Insulin Receptor Substrate 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IRS1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IRS1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IRS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IRS1. You can calculate the concentration of Human IRS1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
88-98
97-111
Average (%)
102
93
102
1:4
Range (%)
87-101
80-93
86-98
Average (%)
93
85
92
1:8
Range (%)
90-104
82-95
84-93
Average (%)
96
87
88
1:16
Range (%)
92-107
79-89
84-94
Average (%)
98
85
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
93-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.11
4.85
0.48
1.1
4.8
Standard deviation
0.03
0.06
0.15
0.03
0.06
0.2
C V (%)
5.88
5.41
3.09
6.25
5.45
4.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human IRS1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human IRS1 in samples. No significant cross-reactivity or interference between Human IRS1 and analogues was observed.
