Human ISG15 (Interferon Stimulated Gene 15) ELISA Kit
The Human ISG15 (Interferon-Stimulated Gene 15) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ISG15 levels in human serum, plasma, and cell culture supernatants. This kit delivers reliable and reproducible results, making it ideal for a variety of research applications.ISG15 is a key protein in the interferon pathway, playing a crucial role in the host immune response to viral infections and other pathogens. Dysregulation of ISG15 has been implicated in various diseases, including autoimmune disorders, infectious diseases, and cancer.
Therefore, the Human ISG15 ELISA Kit is an essential tool for studying these conditions and potentially developing novel therapeutic interventions.Overall, the Human ISG15 ELISA Kit offers researchers a valuable tool for investigating the role of ISG15 in health and disease, providing accurate and precise measurements for insightful research purposes.
Product Name:
Human ISG15 (Interferon Stimulated Gene 15) ELISA Kit
SKU:
HUES01468
Target:
Human ISG15 (Interferon Stimulated Gene 15)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ISG15. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ISG15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ISG15, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ISG15. You can calculate the concentration of Human ISG15 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
85-98
86-100
Average (%)
101
91
92
1:4
Range (%)
92-104
84-95
83-94
Average (%)
98
89
89
1:8
Range (%)
93-107
87-100
82-95
Average (%)
100
92
88
1:16
Range (%)
92-106
81-96
84-96
Average (%)
98
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
99.1
288.84
966.26
89.56
303.51
1026.46
Standard deviation
6.05
11.81
51.41
5.9
12.44
40.44
C V (%)
6.1
4.09
5.32
6.59
4.1
3.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human ISG15 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ISG15 in samples. No significant cross-reactivity or interference between Human ISG15 and analogues was observed.
