Human Isocitrate Dehydrogenase 2,mitochondrial (IDH2) ELISA Kit
The Human Isocitrate Dehydrogenase 2 (Mitochondrial) (IDH2) ELISA Kit is a highly reliable and sensitive assay designed for the accurate measurement of IDH2 levels in human serum, plasma, and cell culture supernatants. This kit provides researchers with the ability to analyze IDH2 levels with high specificity, ensuring trustworthy and consistent results for a variety of research applications.IDH2 is a key enzyme involved in the tricarboxylic acid cycle, playing a crucial role in cellular energy production and metabolism.
Mutations in the IDH2 gene have been linked to various diseases, including cancer and metabolic disorders, making it a valuable biomarker for studying these conditions and exploring potential treatment options.By using the Human Isocitrate Dehydrogenase 2 (Mitochondrial) (IDH2) ELISA Kit, researchers can gain valuable insights into the role of IDH2 in health and disease, paving the way for advancements in diagnosis, prognosis, and therapeutic development.
Product Name:
Human Isocitrate Dehydrogenase 2,mitochondrial (IDH2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
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