The Human ITLN1 (Intelectin-1/Omentin) CLIA Kit is specifically designed for the precise detection of ITLN1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for various research applications. ITLN1, also known as Intelectin-1 or Omentin, is a protein that plays a critical role in immune response and metabolic regulation. It is associated with conditions such as obesity, diabetes, and cardiovascular diseases, making it a potential biomarker for studying and developing treatments for these health issues.
Whether you are studying immune function, metabolic disorders, or cardiovascular health, the Human ITLN1 CLIA Kit provides a reliable and efficient solution for measuring ITLN1 levels in human samples. With its user-friendly protocol and excellent performance, this kit is a valuable addition to any research laboratory.
Product Name:
Human ITLN1 (Intelectin 1/Omentin) CLIA Kit
SKU:
HUES01092
Target:
Human ITLN1 (Intelectin 1/Omentin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ITLN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ITLN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ITLN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ITLN1. You can calculate the concentration of Human ITLN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-101
102-115
94-108
Average (%)
94
109
101
1:4
Range (%)
96-110
98-115
100-113
Average (%)
101
106
107
1:8
Range (%)
84-99
97-112
93-106
Average (%)
91
103
99
1:16
Range (%)
95-107
98-112
100-114
Average (%)
100
104
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
107
EDTA plasma (n=5)
85-95
90
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
368.73
841.43
3720.56
335.76
867.97
3433.36
Standard deviation
41.85
100.3
231.05
32.43
72.48
270.55
C V (%)
11.35
11.92
6.21
9.66
8.35
7.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ITLN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ITLN1 in samples. No significant cross-reactivity or interference between Human ITLN1 and analogues was observed.
