The Human JAK1 (Janus Kinase 1) ELISA Kit is specifically designed for the precise measurement of JAK1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.JAK1 is a key enzyme in the JAK-STAT signaling pathway, playing a critical role in regulating immune responses and cell growth.
Dysregulation of JAK1 has been linked to autoimmune disorders, inflammatory diseases, and certain cancers, highlighting its significance as a potential diagnostic biomarker and therapeutic target.With its advanced technology and reliable performance, the Human JAK1 ELISA Kit is an invaluable tool for researchers studying the role of JAK1 in various diseases and drug development efforts.
Product Name:
Human JAK1 (Janus Kinase 1) ELISA Kit
SKU:
HUES03528
Target:
Human JAK1 (Janus Kinase 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human JAK1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human JAK1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human JAK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human JAK1. You can calculate the concentration of Human JAK1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
86-100
88-101
Average (%)
93
92
93
1:4
Range (%)
95-106
81-92
84-99
Average (%)
100
86
90
1:8
Range (%)
94-105
87-98
87-99
Average (%)
99
93
93
1:16
Range (%)
88-102
85-97
86-99
Average (%)
95
91
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
88-102
95
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.02
3.44
0.53
0.93
3.44
Standard deviation
0.03
0.05
0.16
0.04
0.05
0.17
C V (%)
6.12
4.9
4.65
7.55
5.38
4.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human JAK1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human JAK1 in samples. No significant cross-reactivity or interference between Human JAK1 and analogues was observed.
