Human Junctional Adhesion Molecule 3 (JAM3) ELISA Kit
The Human Junctional Adhesion Molecule-3 (JAM3) ELISA Kit, which is designed for the accurate detection of JAM3 levels in human samples. This kit offers high sensitivity and specificity, allowing for reliable and reproducible results in a variety of research applications. Junctional adhesion molecule-3 (JAM3) is a key protein involved in cell adhesion and migration, playing a crucial role in various physiological and pathological processes. Studying JAM3 levels can provide valuable insights into inflammatory responses, immune cell trafficking, and tissue regeneration, making it a valuable tool for researchers studying these areas of interest.
The Human Junctional Adhesion Molecule-3 (JAM3) ELISA Kit is a valuable resource for researchers looking to investigate the role of JAM3 in human health and disease. Its high performance and accuracy make it a reliable choice for labs conducting research in areas such as immunology, cell biology, and vascular biology.
Product Name:
Human Junctional Adhesion Molecule 3 (JAM3) ELISA Kit
Product Code:
HUDL01589
Size:
96 Assays
Target:
JAM3
Synonyms:
JAM-C, JAMC
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
