Human Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit
The Human KEAP1 (Kelch-like ECH-associated protein 1) ELISA Kit is specifically designed for the precise measurement of KEAP1 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for various research applications.KEAP1 is a key protein involved in the regulation of oxidative stress response and plays a crucial role in maintaining cellular homeostasis.
Dysregulation of KEAP1 has been linked to various diseases, including cancer, neurodegenerative disorders, and respiratory diseases, highlighting its significance as a potential biomarker for disease investigation and therapeutic development.Overall, the Human KEAP1 ELISA Kit offers researchers a reliable and efficient method for studying the role of KEAP1 in health and disease, paving the way for advancements in biomedical research and clinical diagnostics.
Product Name:
Human Kelch Like ECH Associated Protein 1 (KEAP1) ELISA Kit
Product Code:
HUDL01603
Size:
96 Assays
Synonyms:
INrf2, KLHL19, Cytosolic inhibitor of Nrf2, Kelch-like protein 19
Detection Method:
Sandwich
Range:
31.2-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
