The Human Keratin 14 (KRT14) ELISA Kit is a reliable tool for the precise measurement of keratin 14 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring accurate and reproducible results for various research applications.Keratin 14 is a key protein involved in maintaining the structural integrity of epithelial cells, particularly in the skin and other epithelial tissues. Abnormal expression of keratin 14 has been linked to various skin disorders, including dermatoses and carcinomas.
As such, monitoring keratin 14 levels can provide valuable insights into the pathogenesis of these diseases and potential therapeutic targets.With its high performance and ease of use, the Human Keratin 14 (KRT14) ELISA Kit is an indispensable tool for researchers studying epithelial biology, skin diseases, and cancer. Stay ahead in your research with this advanced ELISA kit from Assay Genie.
Product Name:
Human Keratin 14 (KRT14) ELISA Kit
Product Code:
HUDL01646
Size:
96 Assays
Target:
KRT14
Synonyms:
CK14, EBS3, EBS4, K14, Cytokeratin 14, Epidermolysis Bullosa Simplex Dowling-Meara Koebner, Keratin, type I cytoskeletal 14
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
