The Human Keratin 16 (KRT16) ELISA Kit is specifically designed for the precise measurement of KRT16 levels in human tissue and cell lysates. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results for a variety of research purposes.Keratin 16 is a key protein that plays a critical role in the structure and function of epithelial cells, particularly in the skin, hair, and nails. Dysregulation of KRT16 expression is implicated in various dermatological conditions, including psoriasis, eczema, and skin cancer, underscoring its importance as a valuable marker for investigating these disorders and developing potential treatments.
With its reliable performance and broad applicability, the Human Keratin 16 (KRT16) ELISA Kit is an indispensable tool for researchers studying the role of KRT16 in epithelial biology and skin diseases.
Product Name:
Human Keratin 16 (KRT16) ELISA Kit
Product Code:
HUDL01647
Size:
96 Assays
Target:
KRT16
Synonyms:
CK16, NEPPK, Cytokeratin 16, Focal Non-Epidermolytic Palmoplantar Keratoderma, Keratin, type I cytoskeletal 16
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
