The Human Keratin 4 (KRT4) ELISA Kit is specially designed for the precise quantification of KRT4 levels in human samples such as serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research applications. Keratin 4 is a protein that plays a vital role in maintaining the structural integrity of epithelial cells, particularly in the skin and mucous membranes.
Dysregulation of KRT4 expression has been linked to various skin disorders, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions. With the Human Keratin 4 ELISA Kit, researchers can easily and efficiently measure KRT4 levels in human samples, providing valuable insights into the role of this protein in various pathological conditions and paving the way for new discoveries in skin biology and dermatology.
Product Name:
Human Keratin 4 (KRT4) ELISA Kit
Product Code:
HUDL01656
Size:
96 Assays
Target:
KRT4
Synonyms:
CYK4, CK4, K4, Cytokeratin 4, Keratin, type II cytoskeletal 4, Type-II keratin Kb4
Detection Method:
Sandwich
Range:
0.156-10ng/mL.
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
