Human Kinesin Family, Member 18A (KIF18A) ELISA Kit
The Human KIF18A (Kinesin Family Member 18A) ELISA Kit is a specialized assay designed for the precise measurement of KIF18A levels in human serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring consistent and dependable results that are ideal for a wide range of research applications.KIF18A is a crucial member of the kinesin superfamily of motor proteins, playing a key role in chromosome alignment and segregation during cell division.
Dysregulation of KIF18A expression has been linked to various diseases, including cancer and developmental disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Human KIF18A ELISA Kit offers researchers a powerful tool for studying the function and regulation of KIF18A, providing valuable insights into its role in health and disease.
Product Name:
Human Kinesin Family, Member 18A (KIF18A) ELISA Kit
Product Code:
HUDL01607
Size:
96 Assays
Target:
KIF18A
Synonyms:
MS-KIF18A, Marrow stromal KIF18A, Kinesin-like protein KIF18A
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-102
93
EDTA plasma (n=5)
91-100
95
Heparin plasma (n=5)
92-98
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
