The Human KLK7 (Kallikrein 7) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of KLK7 levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.KLK7 is an important serine protease involved in various biological processes such as skin barrier function, inflammation, and cancer progression.
Abnormal levels of KLK7 have been linked to various diseases, including skin disorders and certain types of cancer. Therefore, this ELISA kit serves as a valuable tool for studying KLK7 biology and its potential role in disease development, as well as for identifying potential therapeutic targets.
Product Name:
Human KLK7 (Kallikrein 7) ELISA Kit
SKU:
HUES01699
Target:
Human KLK7 (Kallikrein 7)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human KLK7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KLK7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human KLK7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human KLK7. You can calculate the concentration of Human KLK7 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-101
87-103
95-112
Average (%)
92
94
102
1:4
Range (%)
89-103
82-92
82-95
Average (%)
95
87
88
1:8
Range (%)
91-103
84-98
83-93
Average (%)
97
91
88
1:16
Range (%)
86-101
85-99
83-93
Average (%)
93
90
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
85-97
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.38
3.62
0.44
1.38
3.58
Standard deviation
0.03
0.07
0.11
0.02
0.07
0.15
C V (%)
6.52
5.07
3.04
4.55
5.07
4.19
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human KLK7 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human KLK7 in samples. No significant cross-reactivity or interference between Human KLK7 and analogues was observed.
