The Human KLF4 ELISA Kit is specifically designed for the precise measurement of Kruppel-like factor 4 (KLF4) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.KLF4 is a transcription factor that plays a critical role in regulating cell differentiation, proliferation, and maintenance of stem cell pluripotency. Dysregulation of KLF4 expression has been linked to various diseases, including cancer, inflammatory disorders, and cardiovascular conditions.
As a key molecular target, monitoring KLF4 levels can provide valuable insights into disease progression and potential therapeutic interventions.With its reliable performance and ease of use, the Human KLF4 ELISA Kit is an indispensable tool for researchers studying the functions and implications of KLF4 in health and disease. Get your kit today to advance your research efforts and uncover novel insights into KLF4 biology.
Product Name:
Human Kruppel Like Factor 4, Gut (KLF4) ELISA Kit
Product Code:
HUDL01620
Size:
96 Assays
Target:
KLF4
Synonyms:
EZF, GKLF, Gut-Enriched Krüppel-Like Factor, Epithelial zinc finger protein EZF
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
