Human L1CAM/CD171 (L1-Cell Adhesion Molecule) CLIA Kit
The Human L1CAM (CD171) L1 Cell Adhesion Molecule CLIA Kit is a cutting-edge assay designed for the accurate detection of L1CAM levels in human samples. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.L1CAM, also known as CD171, plays a crucial role in cell adhesion and migration, making it a key protein in processes such as neuronal growth and cancer metastasis.
By measuring L1CAM levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Whether studying neurological disorders, cancer progression, or other conditions involving cell adhesion, the Human L1CAM (CD171) L1 Cell Adhesion Molecule CLIA Kit is an indispensable tool for reliable biomarker analysis and research advancement.
Product Name:
Human L1CAM/CD171 (L1-Cell Adhesion Molecule) CLIA Kit
SKU:
HUES01044
Target:
Human L1CAM/CD171 (L1-Cell Adhesion Molecule)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human L1CAM/CD171. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human L1CAM/CD171 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human L1CAM/CD171, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human L1CAM/CD171. You can calculate the concentration of Human L1CAM/CD171 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-98
94-110
89-102
Average (%)
90
101
94
1:4
Range (%)
96-111
95-110
100-117
Average (%)
102
101
108
1:8
Range (%)
84-99
98-111
102-117
Average (%)
91
104
110
1:16
Range (%)
91-107
99-112
92-105
Average (%)
99
107
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
100.19
252.0
962.69
106.71
270.37
917.25
Standard deviation
10.06
25.45
99.73
9.87
31.58
90.81
C V (%)
10.04
10.1
10.36
9.25
11.68
9.9
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human L1CAM/CD171 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human L1CAM/CD171 in samples. No significant cross-reactivity or interference between Human L1CAM/CD171 and analogues was observed.
