Human LAB7-2/CD86 (B-Lymphocyte Activation Antigen B7-2) CLIA Kit
The Human CD86 (B Lymphocyte Activation Antigen B7-2) CLIA Kit is specifically designed for the quantitative measurement of CD86 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.CD86, also known as B7-2, is a critical co-stimulatory molecule involved in the activation of B lymphocytes and T lymphocytes. It plays a crucial role in regulating the immune response and is essential for the proper functioning of the immune system.
Abnormalities in CD86 expression have been linked to various autoimmune diseases, allergies, and cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human CD86 CLIA Kit provides researchers with a reliable tool for studying the role of CD86 in immune regulation and disease pathogenesis.
Product Name:
Human LAB7-2/CD86 (B-Lymphocyte Activation Antigen B7-2) CLIA Kit
SKU:
HUES00924
Target:
Human LAB7-2/CD86 (B-Lymphocyte Activation Antigen B7-2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2/CD86. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LAB7-2/CD86 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LAB7-2/CD86, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LAB7-2/CD86. You can calculate the concentration of Human LAB7-2/CD86 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
85-99
86-96
Average (%)
98
92
91
1:4
Range (%)
95-106
92-106
99-115
Average (%)
100
98
106
1:8
Range (%)
92-103
85-97
96-111
Average (%)
97
92
101
1:16
Range (%)
85-99
101-119
101-115
Average (%)
92
109
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-104
98
EDTA plasma (n=5)
98-111
105
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.2
193.36
818.3
88.14
185.49
859.15
Standard deviation
11.05
15.43
59.0
7.21
14.65
61.26
C V (%)
12.12
7.98
7.21
8.18
7.9
7.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LAB7-2/CD86 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LAB7-2/CD86 in samples. No significant cross-reactivity or interference between Human LAB7-2/CD86 and analogues was observed.
