The Human Laminin Receptor 1 (LAMR1) ELISA Kit is specifically designed for the precise quantitative measurement of LAMR1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications. LAMR1 is a key receptor protein involved in cell adhesion, migration, and signaling processes. It plays a critical role in various physiological and pathological conditions, including cancer, inflammation, and infectious diseases.
Detecting and monitoring LAMR1 levels can provide valuable insights into disease progression and potential therapeutic targets. Researchers and clinicians can rely on the Human Laminin Receptor 1 (LAMR1) ELISA Kit for robust and reliable quantification of LAMR1 levels, enabling advanced studies and investigations in the fields of oncology, immunology, and cell biology. Trust in the accuracy and precision of this ELISA kit to enhance your research outcomes and discoveries in the study of LAMR1-mediated pathways and biological processes.
Product Name:
Human Laminin Receptor 1 (LAMR1) ELISA Kit
Product Code:
HUDL01681
Size:
96 Assays
Target:
LAMR1
Synonyms:
RPSA, LRP, 37LRP, p40, 40S ribosomal protein SA, 37 kDa laminin receptor precursor, Colon carcinoma laminin-binding protein, Multidrug resistance-associated protein MGr1-Ag
Detection Method:
Sandwich
Range:
78.125-5,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
