Human LBP (Lipopolysaccharide Binding Protein) CLIA Kit
The Human LBP (Lipopolysaccharide Binding Protein) CLIA Kit is specifically designed for the accurate measurement of LBP levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and trustworthy results for a variety of research purposes.Lipopolysaccharide Binding Protein (LBP) is a key component in the immune response to bacterial infections, serving as a vital mediator in the recognition and neutralization of bacterial lipopolysaccharides.
Elevated levels of LBP have been associated with inflammatory conditions, sepsis, and other infectious diseases, making it a valuable biomarker for monitoring and studying these pathological processes.Overall, the Human LBP CLIA Kit provides researchers with a reliable tool to investigate the role of LBP in various disease states and to potentially develop targeted therapeutic interventions.
Product Name:
Human LBP (Lipopolysaccharide Binding Protein) CLIA Kit
SKU:
HUES01191
Target:
Human LBP (Lipopolysaccharide Binding Protein)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LBP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LBP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LBP. You can calculate the concentration of Human LBP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-114
88-102
89-105
Average (%)
104
94
96
1:4
Range (%)
94-106
99-114
99-113
Average (%)
100
106
105
1:8
Range (%)
88-100
92-104
86-98
Average (%)
93
97
91
1:16
Range (%)
86-99
86-99
94-109
Average (%)
92
93
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
106
EDTA plasma (n=5)
97-110
104
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.88
4.39
19.45
1.73
4.5
18.42
Standard deviation
0.2
0.46
1.87
0.19
0.35
1.27
C V (%)
10.64
10.48
9.61
10.98
7.78
6.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LBP in samples. No significant cross-reactivity or interference between Human LBP and analogues was observed.
