Human LBP (Lipopolysaccharide Binding Protein) ELISA Kit
The Human Lipopolysaccharide Binding Protein (LBP) ELISA Kit is carefully crafted for the precise quantitation of lipopolysaccharide binding protein in a variety of human biological samples. LBP is a critical component of the innate immune response, where it plays a key role in recognizing and neutralizing bacterial lipopolysaccharides, thereby aiding in the body's defense against infection and inflammation. Accurate measurement of LBP levels is vital for studying host-pathogen interactions, infectious diseases, and inflammatory conditions.
Our LBP ELISA Kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results to support your research endeavors. Manufactured under strict quality control measures, this kit delivers reliable performance and ease of use, making it an ideal tool for investigative studies. Trust Assay Genie's LBP ELISA Kit for robust quantification of this important biomarker in your research.
Product Name:
Human LBP (Lipopolysaccharide Binding Protein) ELISA Kit
SKU:
AEES00141
Target:
Human LBP (Lipopolysaccharide Binding Protein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LBP. You can calculate the concentration of Human LBP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-105
88-97
96-110
Average (%)
99
92
103
1:4
Range (%)
88-100
87-103
98-108
Average (%)
96
95
105
1:8
Range (%)
87-98
93-105
99-109
Average (%)
93
97
105
1:16
Range (%)
92-98
88-100
87-95
Average (%)
95
96
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-108
104
EDTA plasma (n=5)
87-96
93
Cell culture media (n=5)
92-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.26
6.09
24.73
2.45
6.22
21.54
Standard deviation
0.12
0.28
1.24
0.13
0.28
1.14
C V (%)
5.31
4.6
5.01
5.31
4.5
5.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LBP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LBP in samples. No significant cross-reactivity or interference between Human LBP and analogues was observed.
