Human LCA/CD45 ( Leukocyte Common Antigen) ELISA Kit
The Human LCA/CD45 (Leukocyte Common Antigen) ELISA Kit is specifically designed for the accurate detection of LCA/CD45 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.LCA/CD45 is a critical marker for the identification and characterization of leukocytes, playing a crucial role in immune cell function and response.
Dysregulation of LCA/CD45 expression has been associated with various immune-related disorders, making it a valuable biomarker for studying immune system function and disease pathogenesis.With its superior performance and ease of use, the Human LCA/CD45 ELISA Kit is an essential tool for researchers studying immune responses, inflammation, and immunological disorders. Get accurate and reliable results with this innovative ELISA kit from Assay Genie.
Product Name:
Human LCA/CD45 ( Leukocyte Common Antigen) ELISA Kit
SKU:
HUES02140
Target:
Human LCA/CD45 ( Leukocyte Common Antigen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LCA/CD45. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LCA/CD45 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LCA/CD45, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LCA/CD45. You can calculate the concentration of Human LCA/CD45 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-110
88-99
94-106
Average (%)
103
93
101
1:4
Range (%)
89-103
84-99
83-96
Average (%)
96
90
89
1:8
Range (%)
91-103
86-96
88-102
Average (%)
96
91
94
1:16
Range (%)
91-104
87-100
88-100
Average (%)
96
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
95-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
0.91
4.33
0.48
0.83
3.9
Standard deviation
0.04
0.05
0.19
0.02
0.03
0.19
C V (%)
7.55
5.49
4.39
4.17
3.61
4.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LCA/CD45 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LCA/CD45 in samples. No significant cross-reactivity or interference between Human LCA/CD45 and analogues was observed.
