The Human LCN1 (Lipocalin 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of Lipocalin 1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it suitable for a variety of research applications.Lipocalin 1 is a protein that plays a critical role in innate immunity and inflammation, serving as a carrier for small hydrophobic molecules and functioning in various physiological processes.
Dysregulation of Lipocalin 1 has been implicated in a range of diseases including inflammatory disorders, kidney diseases, and even cancer, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human LCN1 CLIA Kit provides researchers with a powerful tool for investigating the role of Lipocalin 1 in health and disease, offering precise and accurate measurement of protein levels in human samples.
Product Name:
Human LCN1 (Lipocalin 1) CLIA Kit
SKU:
HUES00328
Target:
Human LCN1 (Lipocalin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LCN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LCN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LCN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LCN1. You can calculate the concentration of Human LCN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
97-110
89-102
Average (%)
105
103
94
1:4
Range (%)
95-112
90-104
100-116
Average (%)
103
97
108
1:8
Range (%)
101-113
101-119
98-111
Average (%)
107
109
105
1:16
Range (%)
94-104
100-113
92-106
Average (%)
99
106
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
88-101
94
Cell culture media (n=5)
101-116
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.56
4.8
0.5
1.6
4.83
Standard deviation
0.06
0.11
0.35
0.06
0.19
0.39
C V (%)
11.11
7.05
7.29
12.0
11.88
8.07
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LCN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LCN1 in samples. No significant cross-reactivity or interference between Human LCN1 and analogues was observed.
