The Human LDL (Low Density Lipoprotein) CLIA Kit is specifically designed for the accurate measurement of LDL levels in human serum samples. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for research applications related to cardiovascular health and lipid metabolism.LDL, commonly known as "bad cholesterol," is a key biomarker for assessing cardiovascular risk and the development of atherosclerosis. Elevated LDL levels are associated with an increased risk of heart disease and stroke, making it essential for monitoring and managing cardiovascular health.
By accurately quantifying LDL levels, researchers can gain valuable insights into the impact of diet, lifestyle, and genetics on cardiovascular health, as well as evaluate the effectiveness of therapeutic interventions aimed at lowering LDL levels.Overall, the Human LDL CLIA Kit offers researchers a reliable tool to study the role of LDL in cardiovascular disease and develop targeted strategies for preventing and treating cardiovascular conditions.
Product Name:
Human LDL (Low Density Lipoprotein) CLIA Kit
SKU:
HUES00691
Target:
Human LDL (Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LDL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LDL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LDL. You can calculate the concentration of Human LDL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-118
97-112
102-119
Average (%)
109
104
109
1:4
Range (%)
101-115
100-115
94-108
Average (%)
108
106
101
1:8
Range (%)
100-114
92-105
96-109
Average (%)
108
98
103
1:16
Range (%)
98-114
101-115
88-102
Average (%)
104
108
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-106
100
EDTA plasma (n=5)
86-97
92
Cell culture media (n=5)
101-113
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
5.2
12.21
42.91
5.6
12.54
40.03
Standard deviation
0.55
0.9
4.16
0.46
1.36
3.28
C V (%)
10.58
7.37
9.69
8.21
10.85
8.19
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LDL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LDL in samples. No significant cross-reactivity or interference between Human LDL and analogues was observed.
