The LEP (Leptin) ELISA Kit is specially formulated for the precise and quantitative detection of Leptin levels in various human biological samples. Leptin, a crucial hormone produced by adipose tissue, regulates energy balance, metabolism, and appetite. Its key role in metabolic processes and signaling makes it an essential biomarker for understanding metabolic disorders and obesity-related conditions. With exceptional sensitivity and specificity, the LEP ELISA Kit offers reliable and reproducible results.
Manufactured to meet stringent quality control standards, this kit ensures robust performance and user-friendly operation, catering to both research and clinical applications. Put your trust in Assay Genie's LEP ELISA Kit for accurate and dependable quantification of this fundamental biomarker in your research studies.
Product Name:
Human LEP (Leptin) ELISA Kit
SKU:
AEES00053
Target:
Human LEP (Leptin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LEP. You can calculate the concentration of Human LEP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-95
100-109
95-104
Average (%)
91
105
99
1:4
Range (%)
85-96
86-96
91-103
Average (%)
93
91
97
1:8
Range (%)
91-104
90-99
84-97
Average (%)
97
93
91
1:16
Range (%)
88-96
92-103
94-102
Average (%)
92
100
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
93-104
96
Cell culture media (n=5)
92-100
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
41.5
112.9
459.52
43.08
120.81
467.85
Standard deviation
2.32
5.53
19.21
2.22
5.8
21.47
C V (%)
5.59
4.9
4.18
5.15
4.8
4.59
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human LEP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LEP in samples. No significant cross-reactivity or interference between Human LEP and analogues was observed.
