The Human LEPRECAN-Like Protein 1 (LEPREL1) ELISA Kit is a comprehensive tool for detecting levels of LEPREL1 in human samples. This kit offers exceptional sensitivity and specificity, enabling researchers to obtain accurate and consistent results for various research purposes.LEPREL1 is a key protein involved in collagen synthesis and extracellular matrix formation, playing a crucial role in maintaining tissue structure and integrity. Dysregulation of LEPREL1 has been linked to various diseases, including osteogenesis imperfecta and other connective tissue disorders.
The ability to measure LEPREL1 levels accurately can provide valuable insights into disease mechanisms and potential therapeutic strategies.The Human LEPRECAN-Like Protein 1 (LEPREL1) ELISA Kit is an indispensable tool for researchers studying the role of LEPREL1 in health and disease. With its high sensitivity and specificity, this kit offers reliable and reproducible results, making it a valuable asset for a wide range of research applications.
Product Name:
Human Leprecan Like Protein 1 (LEPREL1) ELISA Kit
Product Code:
HUDL01707
Size:
96 Assays
Target:
LEPREL1
Synonyms:
MLAT4, P3H2, Prolyl 3-Hydroxylase 2, Myxoid liposarcoma-associated protein 4
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
