Human Leucine Rich Repeat Containing Protein 4B (LRRC4B) ELISA Kit
The Human Leucine-Rich Repeat Containing Protein 4B (LRRC4B) ELISA Kit is a powerful tool for the accurate quantification of LRRC4B levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.LRRC4B is a protein that contains leucine-rich repeats, which are known to be involved in protein-protein interactions. This protein is believed to play a role in cell adhesion and migration, making it a key player in processes such as development and immune response.
Dysregulation of LRRC4B has been implicated in various diseases, making it a valuable biomarker for research and potential therapeutic targets.With its precision and accuracy, the Human LRRC4B ELISA Kit is an essential tool for researchers studying cell adhesion, migration, and related processes, as well as those exploring the role of LRRC4B in disease pathways. Its user-friendly design and comprehensive instructions make it suitable for both experienced researchers and newcomers to the field. Unlock the potential of LRRC4B research with this cutting-edge ELISA Kit from AssayGenie.
Product Name:
Human Leucine Rich Repeat Containing Protein 4B (LRRC4B) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
