SynonymsCD85k,LILR-B4,LILRB5,HM18,ILT3,LIR-5,Immunoglobulin-like transcript 3,Leukocyte immunoglobulin-like receptor 5,CD85 antigen-like family member K
ReactivityHuman
Sample TypeTissue Homogenates, Cell Lysates And Other Biological Fluids
SKU:
HUDL01718
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8NHJ6
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD85k,LILR-B4,LILRB5,HM18,ILT3,LIR-5,Immunoglobulin-like transcript 3,Leukocyte immunoglobulin-like receptor 5,CD85 antigen-like family member K
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4) ELISA Kit
The Human Leukocyte Immunoglobulin-like Receptor Subfamily B Member 4 (LILRB4) ELISA Kit is a valuable tool for the precise measurement of LILRB4 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, providing researchers with reliable and consistent results for a variety of investigative purposes.LILRB4 is a key receptor involved in the regulation of immune responses, particularly in the context of immune tolerance and inflammation.
Its dysregulation has been linked to autoimmune diseases, cancer, and infectious diseases, highlighting its importance as a potential diagnostic marker and therapeutic target.By utilizing the Human LILRB4 ELISA Kit, researchers can gain insights into the role of LILRB4 in various diseases and immune processes, ultimately leading to the development of novel treatment strategies and personalized medicine approaches.
Product Name:
Human Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4) ELISA Kit
Product Code:
HUDL01718
Size:
96 Assays
Target:
LILRB4
Synonyms:
CD85k, LILR-B4, LILRB5, HM18, ILT3, LIR-5, Immunoglobulin-like transcript 3, Leukocyte immunoglobulin-like receptor 5, CD85 antigen-like family member K
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
