Human LIFR (Leukemia Inhibitory Factor Receptor) CLIA Kit
The Human LIFR (Leukemia Inhibitory Factor Receptor) CLIA Kit is specifically designed for the accurate measurement of LIFR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.LIFR is an important receptor protein involved in the regulation of cell growth and differentiation, playing a crucial role in various physiological processes. Dysregulation of LIFR has been linked to conditions such as leukemia, inflammatory diseases, and neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and proven performance, the Human LIFR CLIA Kit provides researchers with a powerful tool for studying the role of LIFR in health and disease, advancing our understanding of complex biological mechanisms and potentially leading to the development of novel treatment strategies.
Product Name:
Human LIFR (Leukemia Inhibitory Factor Receptor) CLIA Kit
SKU:
HUES00610
Target:
Human LIFR (Leukemia Inhibitory Factor Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LIFR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LIFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LIFR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LIFR. You can calculate the concentration of Human LIFR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
90-102
86-99
Average (%)
104
96
91
1:4
Range (%)
97-113
92-104
102-118
Average (%)
103
98
108
1:8
Range (%)
87-101
88-100
98-115
Average (%)
94
94
106
1:16
Range (%)
103-117
87-99
99-116
Average (%)
109
94
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-104
96
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
85-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
184.46
526.76
1760.55
193.21
550.96
1629.44
Standard deviation
18.59
50.46
138.91
22.45
64.24
98.09
C V (%)
10.08
9.58
7.89
11.62
11.66
6.02
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LIFR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human LIFR in samples. No significant cross-reactivity or interference between Human LIFR and analogues was observed.
