Human LILRB2 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 2) CLIA Kit
The Human LILRB2 (Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2) CLIA Kit is a reliable and accurate tool for the quantitative measurement of LILRB2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.LILRB2 is a key receptor involved in immune regulation and response, particularly in the modulation of immune cell activation and function. Dysregulation of LILRB2 has been linked to autoimmune diseases, inflammatory disorders, and cancer, highlighting its importance as a potential biomarker for disease monitoring and therapeutic development.
With the Human LILRB2 CLIA Kit, researchers can gain valuable insights into the role of LILRB2 in various health conditions and explore its potential as a target for novel therapeutic strategies. Trust in the precision and accuracy of this kit for robust and meaningful results in your studies.
Product Name:
Human LILRB2 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 2) CLIA Kit
SKU:
HUES00886
Target:
Human LILRB2 (Leukocyte Immunoglobulin Like Receptor Subfamily B Member 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LILRB2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LILRB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LILRB2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human LILRB2. You can calculate the concentration of Human LILRB2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
88-102
92-110
Average (%)
95
94
100
1:4
Range (%)
97-115
99-115
96-110
Average (%)
105
105
101
1:8
Range (%)
95-107
96-108
90-104
Average (%)
102
101
95
1:16
Range (%)
85-97
85-100
101-116
Average (%)
92
91
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-99
92
EDTA plasma (n=5)
101-117
108
Cell culture media (n=5)
91-106
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.76
263.95
925.19
87.48
257.17
1005.66
Standard deviation
7.7
19.56
102.88
8.91
26.31
115.35
C V (%)
8.21
7.41
11.12
10.19
10.23
11.47
Sample type &Sample volume:
Tissue homogenates; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human LILRB2 concentrations in Tissue homogenates.
Specificity:
This kit recognizes Human LILRB2 in samples. No significant cross-reactivity or interference between Human LILRB2 and analogues was observed.
